Domian, I. J., Reisenauer, A., Shapiro, L. Bacterial cell division: A moveable feast, Cell cycle-dependent polar localization of an essential bacterial histidine kinase that controls DNA replication and cell division. Brenda Wu, SURF Scholar 2016 BS Chemistry, Caltech 2019 In at least two alpha subdivision bacteria, R. meliloti and C. crescentus, CcrM-mediated methylation has important cellular functions. The tests have not been cleared or approved by the US Food and Drug Administration (FDA). Entry into the microdomain is selective for cytosolic proteins and requires a binding pathway to PopZ. We propose that polar recruitment of CtrA evolved to ensure that CtrA is degraded only in the stalked half of the predivisional cell, perhaps by localizing a proteolytic adaptor protein to the stalked pole. By screening 69 stress conditions, we find that HipBA2 responds to multiple stress signals through the proteolysis of HipB2 antitoxin by the Lon protease and the release of active HipA2 kinase, revealing a molecular mechanism that allows disparate stress conditions to be sensed and funneled into a single response pathway.IMPORTANCE To overcome various environmental challenges, bacterial cells can enter a physiologically quiescent state, known as dormancy or persistence, which balances growth and viability. Lasker, K., von Diezmann, A., Moerner, W. E., Shapiro, L. Biomolecular Condensates at Bacterial Cell Poles Function to Drive Spatially Restricted Signal Propagation, Multi-Step 2D Protein Crystallization via Structural Changes within an Ordered Lattice. However, these mutants efficiently transported fatty acids and had beta-oxidation enzyme levels comparable with that of the wild type. Bozdemir, E., Vigil, F.A., Bugay, V., Espinoza, L., Chun, S.H., Hobbs, M., Khoury, S., Holstein, D., Sanchez, I., Cavazos, J., Brenner, R., Carver, C.M., Hastings, S.D., Cook, M.E., and. For any questions or concerns, please feel free to reach out by emailing Scott Gerbert at Purucker, M., Bryan, R., Amemiya, K., Ely, B., Shapiro, L. CHARACTERIZATION OF THE PROTEINS OF THE CAULOBACTER-CRESCENTUS FLAGELLAR FILAMENT - PEPTIDE ANALYSIS AND FILAMENT ORGANIZATION. 2023 Natera, Inc. All Rights Reserved. Pilus assembly in CAULOBACTER: crescentus occurs during a short period of the cell cycle and pili are only present at the flagellar pole of the swarmer cell. We further use the locations of PopZ to provide context for localizations of SpmX, a low-copy integral membrane protein sequestered by PopZ as part of a signaling pathway that leads to an asymmetric cell division. Bennett Shapiro is a provider established in Stanford, California and his medical specialization is Orthopaedic Surgery. This joint publication of SLAC and Fermilab is your view into the world of particle physics. Inserting four bases in front of the AUG at the 5' end of dnaX mRNA abolishes translation in the correct frame. Isolation of the full-length ccrM genes from the aquatic bacterium C. crescentus, the soil bacterium R. meliloti, and the intracellular pathogen B. abortus showed that this sequence conservation extends over the entire protein. We show that the cell-cycle timing of CcrM is critical for Caulobacter fitness. Umbarger, M. A., Toro, E., Wright, M. A., Porreca, G. J., Bau, D., Hong, S., Fero, M. J., Zhu, L. J., Marti-Renom, M. A., McAdams, H. H., Shapiro, L., Dekker, J., Church, G. M. Live-cell single-molecule and super-resolution imaging in bacteria.